A rapid screening test for endocrine disrupting chemicals using primary cell culture of the marine medaka

While endocrine disrupting chemicals (EDCs) pose a significant threat to wildlife worldwide, their diverse chemical structures present a major challenge to their detection, particularly since they are present at very low concentrations in the environment. We here report the development of an in vitro system for rapid screening of EDCs, using primary cell cultures (pituitary, ovarian follicular and testicular cells) of the marine medaka (Oryzias melastigma). Pituitary, testis and ovary cell cultures were developed and challenged by environmentally relevant concentrations of three well known EDCs (viz. estradiol, 2,2′,4,4′-tetrabromodiphenyl ether, and 4-n-nonylphenol) as well as hypoxia (which has been shown to be a potent endocrine disruptor). (…)

Anna C.K. Tse, Karen Y.T. Lau, Wei Ge, Rudolf S.S. Wu, Aquatic Toxicology, Volumes 144–145, Pages 50–58, 15 November 2013

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